How does shotgun cloning differ from the clone-by-clone method?.

Answers

Answer 1

No genetic or physical maps of the genome are needed to begin shotgun cloning  and is much faster.

What is Shotgun cloning?

This is a method to duplicate genomic DNA in which it is smashed into small pieces and the fragments are then taken together and cloned into a vector.

This type of cloning however is faster as there are no genetic or physical maps needed to begin the process.

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Related Questions

Climate is defined by all of the following except for:________.
a. the state of the atmosphere at a specific time and place
b. the state of the atmosphere averaged over time at some place
c. governs fresh water supply, type of crops, distribution of plants and animals
d. is commonly a 30 year average of weather elements e. includes extremes in weather

Answers

c.
climate is defined by all of the following except for: governs fresh water supply, type of crops, distribution of plants and animals

Which Term means source of energy?
A. Carbohydrates
B. Carbon
C. Proteins
D. Lipids
E Nucleic acid
Some body please help me

Answers

Answer:

D. Lipids

Explanation:

A lipid is any of various organic compounds that are insoluble in water. They include fats, waxes, oils, hormones, and certain components of membranes and function as energy-storage molecules and chemical messengers.

Lipids are an efficient source of energy (9kcal per gram) that the body stores in relative abundance. They also play an important role as building blocks for cell membranes, hormones, and physiologically active substances.

Which of the following precautions can help prevent mother-to-child HIV transm ensuring that an infected mother eats 10% more calories ensuring that an infected mother uses condoms starting an infected mother on antiretroviral treatment ensuring that an infected mother avoids antiretroviral treatment Question 3

A: Ensuring that an infected mother eats 10% more calories

B:Ensuring that an injected mother uses condoms

C:starting an infected mother on antiretroviral treatment

D:Ensuring that an infected mother avoids antiretroviral treatment​

Answers

Answer:

C.

Explanation:

Antiretroviral treatment is the most effective way to prevent mother to child infections assuming she is already pregnant.

Wearing condoms only protects others

More calories only needs she's going to be feeding the baby more.

Avoidjng treatment only makes it worse thus making baby more susceptible to catching it.

Which activity of E. coli DNA polymerase I is responsible for proofreading the newly synthesized DNA

Answers

Answer:

Find you answer below:

Explanation:

5′ to 3′ exonuclease activity.

helper T cell provide activation to …….

A- the pathogen
B- B and TC cells
C- the bone marrow
D- thymus gland

Answers

[tex]\qquad \qquad\huge \underline{\boxed{\sf Answer}}[/tex]

Here's the Answer ~

The Helper T cell provide activation to B. [ B and TC cells ]

What forms of hydrogen and oxygen are MOST
common on Earth?

A. Solid

B. Gas

C. Liquid

D. Plasma

Answers

Answer:

B

Explanation:

Hydrogen and Oxygen are the most abundant gases, being the first, and third most abundant.

Hope this helps, googIe only wanted to give me explanations on why Hydrogen is the most common element in the universe :/

The form of hydrogen and oxygen that are most common on Earth's surface is known as Gas. Thus, the correct option for this question is B.

What is the difference between hydrogen and oxygen?

The difference between hydrogen and oxygen is that oxygen significantly contains eight electrons and eight protons while hydrogen contains only one electron and one proton. Oxygen is present in 21% of the earth's atmosphere, while hydrogen is present in 0.03% which is negligible.

According to the context of this question, oxygen is the first and most abundant gas in the earth's atmosphere and hydrogen is the third most abundant gas.

They are also present in the liquid and solid states but are in negligible amounts when treated with abnormal conditions like lower temperature and freezing.

Therefore, gaseous forms of hydrogen and oxygen are most common on Earth's surface. Thus, the correct option for this question is B.

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According to the model below, how long ago did the common ancestor of all the organisms on the
phylogenetic tree exist?
Millions of Years Ago

Answers

Answer

about 3.9 billion years ago

about 3.9 billion years agoThe most recent common ancestor of all currently living organisms is the last universal ancestor, which lived about 3.9 billion years ago.

Please help this is due 9 hours
Scientists often use controlled experiments to answer questions.
Choose the three correct statements about controlled experiments.
A) No changes are made to a test group in an experiment.
B) A control group is used for comparison to other groups.
C) Only one change can be made per test group in an experiment.
D) Changes can only be made to the control group in an experiment.
E) A controlled experiment must have a control group and test group(s).

Answers

Answer:

B) A control group is used for comparison to other groups.

E) A controlled experiment must have a control group and test group(s).

C) Only one change can be made per test group in an experiment.

Answer: B, C, E

Explanation B is correct due to the fact the control groups are design to compare the results from the experiments to the control group which is ensures that the experiments actually work

Explanation: C is correct because each experiment group can only have one change made to it that differs from the control group. If you made multiple changes to one group you would not know what change you made that resulting in how your experiment ended up

Explanation E: An experiment is not scientifically proven unless you have a control group hence the need for both test groups and a control group


Cells without a nucleus are

Answers

Answer: prokaryotic cells

     Explanation:

A prokaryotic cell is a cell without a nucleus.  Since this is a simple definition I cannot explain much further, but an example of a prokaryotic cell is bacteria.

A food allergy is a reaction that occurs when the _____ system reacts to a harmless substance in food, such as a protein that does not undergo digestion.

Answers

A food allergy is a reaction that occurs when the body system reacts to a harmless substance in food, such as a protein that does not undergo digestion

1) What do you think made Rachel Carson's book, Silent Spring, so inspiring to readers?

Answers

Because it uncovers a truth many don’t see in science theory’s and thing that people might not have know giving them the curiosity to try something new and learn something new.

hello humans ~
Based on the given statements, choose the correct option.
Statement 1: Amoeba and WBCs use pseudopodia for locomotion.

Statement 2: Amoeba and WBCs are both capable of independent existence.

Statement 3: Amoeba and WBCs can change their shapes.

Statement 4: Amoeba and WBCs have cell membrane.

A. Statement 3 is incorrect.

B. Statement 1 and 2 are incorrect.

C. Statement 1, 2, 3 and 4 are incorrect.

D. Statement 4 is incorrect

Answers

Answer:

amoeba and WBCs can change their shape

Explanation:

Amoeba cells and white blood cells can change their shapes. Amoeba consists of pseudopodia also known as the false feet. White blood cells can engulf or kill pathogens by changing their shape.

Hope it helps!!!brainliest pls!!!

What is the effect of hyperinsulinism on the metabolism of carbohydrates, amino acids, and lipids by the liver

Answers

Hyperinsulinism produces an excessive uptake of glucose by the liver (hypoglycemia). It also leads to an interruption in amino acid and fatty acid catabolism.

What is hyperinsulin?

Hyperinsulin is a condition associated with the excessive production of insulin, a key hormone in glucose metabolism.

The main effect of hyperinsulinism is an excessive uptake of glucose by the liver, which is associated with hypoglycemia.

Hyperinsulin can also produce a chronic interruption in the amino acid and fatty acid metabolism (catabolism).

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How does a possum in Florida adapt to the winter season?

Answers

Answer:

They spend the winter in dens. These dens are dry and safe for them to stay in, while the harsh winter storms rage above.

I hope this answers your question! Have a wonderful day.

Answer:

they find a den dunno how, but they do and wait it out like a wise man.

Explanation:

Inside a muscle fiber, what triggers sarcomeres to contract?.

Answers

Answer:

It would be an Impulse.

Explanation:

When an impulse reaches the muscle fibres of a motor unit, it stimulates a reaction in each sarcomere between the actin and myosin filaments.

Does this help you??

Answer:

A sudden rise in cystolic Ca2+

Which of the following farming approaches can grow food crops with less land use?
a. organic farming
b. vertical farming
c. industrial farming
d. biodynamic farming

Answers

Answer:

B. vertical farming

The farming approach that can grow food crops with less land use is option b, or "vertical farming," as it is an innovative farming approach that involves growing crops in vertically stacked layers.

What is vertical farming?

Vertical farming is an innovative agricultural technique that involves growing crops in vertically stacked layers using artificial lighting and a soilless medium. The plants are grown in a controlled environment where all aspects of the growth process, such as temperature, humidity, and lighting, are carefully managed to optimize plant growth and yields, and vertical farming systems can be located in urban areas or other locations where land is limited or expensive. By growing crops vertically, the amount of land needed to produce a given amount of food is greatly reduced as compared to traditional farming methods that require large amounts of horizontal land.

Hence, the farming approach that can grow food crops with less land use is option b, vertical farming.

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At the end of meiosis I, two cells have been produced.How many replicated chromosomes are in each of these cells?

Answers

Answer:

23

Explanation:

At the end of meiosis-I, two daughter cells are formed having half the number of chromosomes present in diploid cell undergoing meiosis. Each cell has half the number of chromosomes present in the diploid cell.

PLEASE HELP!!!!!!!!! WILL GIVE 60 PTS, NOT A JOKE!!!!


Drop down menus:


In some cases of head injuries, the cerebrum may be affected. This type of injury could cause a loss of _________ and impaired __________.


1) a. breathing regulation

b. reflexive movement

c. voluntary movement


2) a. heart rate

b. involuntary movement

c. speech

Answers

In some cases of head injuries, the cerebrum may be affected. This type of injury could cause a loss of voluntary movement and impaired speech

Gated ion channels for sodium and potassium open and close in rapid succession, causing the neuron to depolarize and then return to its normal resting level, during each:

Answers

Given what we know, we can confirm that this process happens in between each action potential.

What is an action potential?

The action potential is the rapid polarization and depolarization of cells and cell membranes when they receive an impulse. In this case, the action potential is used to open and close the gated ion channels for sodium and potassium.

Therefore, we can confirm that gated ion channels for sodium and potassium open and close in rapid succession, causing the neuron to depolarize and then return to its normal resting level, during each action potential.

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People tint windows dark to encourage __________.
transmission
absorption
reflection
transfusion

Answers

Absorption is the correct answer

Answer:

absorption

Explanation:

dark hues of colour are generally used to absorb heat while since they absorb heat better than white

A sudden, violent, involuntary muscle contraction of a group of muscles caused by a disturbance in brain function is called a ______________________.

Answers

Answer:

seizure

Explanation:

I need help please!!! I swear this isn’t even in my text book!

Answers

Answer:

[tex]\huge\boxed{\sf Option \ B. }[/tex]

Explanation:

Cell Membrane:

are the outermost surface of the cell for animals and secondmost surface of the cell from plants after cell wall.

Surface of the cell membrane:

Cell membrane is partially and selectively permeable which means that it only allows certain substances in and out of the cell.

[tex]\rule[225]{225}{2}[/tex]

The answer is B :) they are semi-permeable

_______, a dystonic reaction that results in muscle spasms in the neck, can be very painful for the client.

Answers

I think, Torticollis (I'm not completely sure)

Hope this helps!

An individual of genotype TtFFgg mates with an individual of genotype ttFfgg. About how many of their 100 offspring would you expect to be heterozygous for all three traits

Answers

Answer:

0

Explanation:

Heterozygous means that they have an allele for both traits and is in the form (Aa) meaning they have both the dominant and recessive trait.

We notice that the third trait, represented by 'g', is in the form gg for both individuals. Since they are both lowercase and the same allele, they are both hom0zygous for that trait, meaning there is no way the offspring can get a dominant trait to make their genotype Gg.

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What are the structures of the circulatory system?.

Answers

Answer:

The circulatory system consists of three independent systems that work together: the heart (cardiovascular), lungs (pulmonary), and arteries, veins, coronary and portal vessels (systemic). The system is responsible for the flow of blood, nutrients, oxygen and other gases, and as well as hormones to and from cells. An average adult has 5 to 6 quarts (4.7 to 5.6 liters) of blood, which is made up of plasma, red blood cells, white blood cells and platelets.


In an ecosystem that is not altered by humans or environmental disasters, a population
will remain close to carrying capacity. This is due to what factors?
Species competition and predator/prey relationships
Drastic decline in producers
Introduction of a new species
Unlimited food and water resource

Answers

Answer:

I think it's Species competition and predator/prey relationships

Explanation:

Hopefully this helps :)

how can the length of a DNA fragment be estimated in gel electrophoresis

Answers

Answer: At the same time as the samples, a DNA marker with known length fragments is routinely passed through the gel. You can estimate the length of the DNA fragments in the samples by comparing the bands of the DNA samples with those of the DNA marker.

Explanation:

similar body structure of a chicken wing? (example: pelvis in fish/cetaceans, feet/)hoof of a horse…)

Answers

Explanation:

body structure of chicken is feather

fill out the following about genetics:

Answers

Answer:

Define alleles:

Alleles are alternate forms of genes that are visible by mutations. They are found at a specific locus in a chromosome.

A. The genotypes of the parents: Ww and ww

B. The genotypic ratio of the offspring: 2:2

C. The phenotypic ratio of the offspring: 2:2

Explanation:

A white-fruited plant is crossed with a yellow-fruited plant. According to the instructions, the allele for white-fruited plants are dominant over the allele for yellow-fruited plants, this means that in order a plant to be white-fruited, they must either have the alleles Ww or WW. In this case, it is better to cross Ww, as all of the offsprings are yellow-fruited (ww).

Whenever we cross Ww and ww, we have a fifty percent chance of the offsprings either having the alleles "Ww" or "ww".

There are four smaller squares in a punnet square, and half of the smaller squares code for the alleles "Ww" and the other half code for the alleles "ww". This means that the genotypic ratio of the offspring would be 2:2, as 2 of the smaller squares code for "Ww", and the other 2 code for "ww".

Since "Ww" has a dominant allele, the phenotype for the organism with this genotype would be white-fruited, and for "ww", the phenotype for the organism would be yellow-fruited. This means that in this case, there would be a 50% chance for a white-fruited plant, and a 50% chance for a yellow-fruited plant. This is why the phenotypic ratio of the offspring would also be 2:2, as half of the smaller squares code for a plant with a white-fruited phenotype, and the other half of the smaller squares code for a plant with a yellow-fruited phenotype.

1.Which type of fresh water is at risk of increased salinity as earth warms?
Glaciers
mountain
streams
coastal
lakes
snowpacks

Answers

Answer: Coastal Lakes

Explanation: I took the assessment:)

The type of freshwater that is at risk of increased salinity as the earth warms is :  Coastal lakes

What are coastal lakes ?

Coastal lakes are water bodies which occur in Basins and are separated from larger water bodies such as oceans and seas by a narrow lands. They run parallel to the shore are mostly likely at the risk of increased salinity as the earth warms and also due to their proximity to salty waters.

Hence we conclude that The type of freshwater that is at risk of increased salinity as the earth warms is :  Coastal lakes.

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